Cynomolgus monkey SELE Gene cDNA Clone / ORF Clone

SELE cDNA Clone Datasheet

SELE cDNA Clone Product Information

• cDNA Size: 1833
• Gene Synonym: SELE
• Source: Cynomolgus monkey

SELE cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) selectin E DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1150 C/G, 1286 C/G resulting in the amino acid Pro substitution by Ala, Thr substitution by Arg and 978 C/T, 1404 C/T not causing the amino acid variation.
  Please check the sequence information before order.

SELE cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SELE cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SELE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATTGCTT CACAGTTTCT CTCGGCTCTC ACTTTGGTGC TTCTCATTAA AGAGAGTGGA
61       GCCTGGTCTT ACAACACCTC CACAGAAGCT ATGACTTATG ATGAGGCCAG TGCTTATTGC
121      CAGCAAAGGT ACACGCATCT GGTTGCAATT CAAAACAAAG AAGAGATTGA GTACCTAAAC
181      TCCATATTGA GCTATTCACC AAGTTATTAC TGGATTGGAA TCAGAAAAGT CAACAATGTG
241      TGGGTCTGGG TAGGAACCCA GAAACCTTTG ACGGAAGAAG CCAAGAACTG GGCTCCAGGT
301      GAACCCAACA ATAGGCAAAA AGATGAGGAC TGCGTGGAGA TCTACATCAA GAGAGACAAA
361      GATGTGGGCA TGTGGAATGA CGAGCGGTGC AGCAAGAAGA AGCTTGCCCT GTGCTACACA
421      GCTGCCTGTA CCAATACATC CTGCAGTGGC CACGGTGAAT GTGTAGAGAC CATCAATAAT
481      TACACTTGCA AGTGTGACCC TGGCTTCAGT GGACTCGAGT GTGAGCAAAT TGTGAACTGT
541      ACAGCCCTGG AATCCCCTGA GCATGGAAGC CTAGTTTGCA GTCACCCACT GGGAAACTTC
601      AGCTACAGTT CTTCCTGCTC TGTCAGCTGT GATAGGGGCT ACCTGCCAAG CAGCGTGGAG
661      ACCACGCAGT GTATGTCCTC TGGAGAATGG AGTGCTCCTA TTCCAGCCTG CAAGGTGGTT
721      GAGTGTGATG CTGTGACAAA TCCAGCCAAT GGGTTTGTGG AATGTTTCCA AAACCCTGGA
781      AGTTTCCCAT GGAACACAAC CTGTACATTT GACTGTGAAG AAGGATTTGA ACTAATGGGA
841      GCCCAGAGCC TTCAGTGTAC CTCATCTGGG AATTGGGACA ATGAGAAGCC AACGTGTAAA
901      GCCGTGACAT GCAGGGCCAT CCGCCAGCCT CAGAATGGCT CTGTGAGGTG CAGCCATTCC
961      CCTGCTGGAG AGTTCACTTT CAAATCATCC TGCAACTTCA CCTGTGAAGA AGGCTTCATG
1021     TTGCAGGGAG CAGCCCAGGT TGAATGCACC ACTCAAGGGC AGTGGACACA GCAAGTCCCA
1081     GTTTGCGAAG CTTTCCAGTG CACAGCCTTG TCCAACCCCG AGCGCGGCTA CATGAATTGT
1141     CTTCCTAGCG CTTCTGGCAG TTTCCGTAAT GGGTCCAGCT GTGAGTTTTC CTGTGAGCAG
1201     GGATTTGTGT TGAAGGGATC CAAAAGGCTC CAATGTGGCC CCACAGGGGA GTGGGACAAT
1261     GAGAAGCCCA CATGTGAAGC TGTGAGATGT GATGCTGTCC ACCAGCCCCA GAGGGGCTTG
1321     GTGAGGTGTG CTCATTCTCC TATTGGAGAA TTCACCTACA AGTCCTCTTG TGCCTTCAGC
1381     TGTGAGGAAG GATTTGAATT ACATGGATCA ACTCAACTTG AGTGCACATC TCAGGGACAA
1441     TGGACAGAAG AGGTTCCTTC CTGCCAAGTG GTAAAATGTT CAAGCCTAGC AGTTCTGGAA
1501     AAGATCAACA TGAGCTGCAG TGGGGAGCCC GTGTTTGGCA CTGTGTGCAA CTTTGCATGT
1561     CCTGAAGGAT GGAGGCTCAA TGGCTCTGCA GCAATGACAT GTGGTGCCAC AGGACACTGG
1621     TCTGGGATGC TGCCTACCTG TGAAGCTCCC ACTGAGTCCA ACACTCCCTT GGTAGCTGGA
1681     CTTTCTGCTG CTGGACTCTC CCTCCTGACA TTAGCTCCAT TTCTCCTCTG GCTTCGGAAA
1741     TGCTTTCGGA AAGCAAAGAA ATTTGTTCCT GCCAGCAGCT GCCAAAGTCT TGAATCAGAT
1801     GGAAGCTACC AAAAGCCTTC TTACATCCTT TAA

 

Quality Control:cDNA sequencing results is available upon request.