Anti-SECTM1 Antibody

Cat: 12269-RP02
Contact us:
All SECTM1 Reagents
CRO Services
Product recommended

All SECTM1 Reagents
Anti-SECTM1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SECTM1 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human SECTM1
Recombinant Human SECTM1 Protein (Catalog#12269-H08H)
Produced in rabbits immunized with purified, recombinant Human SECTM1 (rh SECTM1; Catalog#10045-H08H; NP_001618.2; Met 1-Ala 526). SECTM1 specific IgG was purified by Human SECTM1 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
PBS, pH7.4, 5% Trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SECTM1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SECTM1 Antibody (Rabbit Polyclonal antibody) Images
Human SECTM1 Immunoprecipitation(IP) 8130

SECTM1 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

2 µL anti-SECTM1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SECTM1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 27 kDa

Observed band size: 17 kDa

Human SECTM1 Western blot (WB) 13480

Anti-SECTM1 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

Anti-SECTM1 Antibody Alternative Names
Anti-K12 Antibody
SECTM1 Background Information

Secreted and transmembrane 1 (SECTM1), also known as K12, is a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein of SECTM family. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The human K12 protein has been shown to be primarily expressed in spleen, prostate, testis, small intestine, and in peripheral blood leukocytes. The K12 protein is expressed on the cell surface in such small amounts as to preclude detection. Alternatively, it may be that K12 on the cell surface is rapidly cleaved to generate a soluble K12 protein. Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. May be involved in thymocyte signaling. It had been suggested a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis. In addition, as a putative natural CD7 ligand, SECTM1/K12 may be responsible for the costimulatory role it plays in T cell activation.

Full Name
secreted and transmembrane 1
  • Leta E, et al. (1995) Production and characterization of the extracellular domain of human CD7 antigen: further evidence that CD7 has a role in T cell signaling. Cell Immunol. 165(1): 101-9.
  • Slentz-Kesler KA, et al. (1998) Identification and characterization of K12 (SECTM1), a novel human gene that encodes a Golgi-associated protein with transmembrane and secreted isoforms. Genomics. 47(3): 327-40.
  • Lyman SD, et al. (2000) Identification of CD7 as a cognate of the human K12 (SECTM1) protein. J Biol Chem. 275(5): 3431-7.
  • Lam GK, et al. (2005) Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma. J Clin Immunol. 25(1): 41-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SECTM1 Antibody Rabbit Polyclonal ELISA 12269-RP01
    Anti-SECTM1 Antibody Mouse Monoclonal ELISA 12269-MM01
    Anti-SECTM1 Antibody Rabbit Monoclonal ELISA 12269-R030
    Anti-SECTM1 Antibody Rabbit Monoclonal ELISA 12269-R106
    info info
    添加购物车成功! 添加购物车失败!