Mouse SDC2 Gene cDNA Clone / ORF Clone

SDC2 cDNA Clone Datasheet

SDC2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008304.2
• cDNA Size: 609
• Gene Synonym: Hspg1; Synd2; AA960457; syndecan-2; 4833414L08Rik; Sdc2
• Source: Mouse

SDC2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus syndecan 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 603 T/C not causing the amino acid variation.

SDC2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SDC2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SDC2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCGCG CGTGGATCCT GCTCACCTTG GGCTTGATGG CCTGTGTGTC CGCAGAGACG
61       AGAACAGAGC TGACATCCGA TAAGGATATG TACCTTGACA ATAGCTCCAT TGAGGAAGCT
121      TCAGGAGTAT ATCCTATTGA TGATGATGAC TATTCTTCTG CCTCAGGCTC AGGAGCTGAT
181      GAAGACATAG AGAGTCCAGT TCTGACAACA TCCCAACTGA TTCCAAGAAT CCCACTCACT
241      AGTGCTGCTT CCCCCAAAGT GGAAACCATG ACGTTGAAGA CACAAAGCAT TACACCTGCT
301      CAGACTGAGT CACCTGAAGA AACTGACAAG GAGGAAGTTG ACATTTCTGA GGCAGAAGAG
361      AAGCTGGGCC CTGCTATAAA AAGCACAGAT GTGTACACGG AGAAACATTC AGACAATCTG
421      TTTAAACGGA CAGAAGTTCT AGCAGCCGTC ATTGCTGGTG GTGTGATCGG CTTTCTCTTT
481      GCCATTTTCC TCATCCTGCT ATTGGTGTAC CGCATGCGGA AGAAAGATGA AGGAAGCTAC
541      GACCTTGGAG AACGCAAACC ATCCAGCGCA GCTTACCAGA AGGCACCCAC TAAGGAGTTT
601      TACGCATAA

 

Quality Control:cDNA sequencing results is available upon request.