SCN2B Protein

Sodium channel, voltage-gated, type II, beta subunit Protein Datasheet

SCN2B Protein Product Information

• Synonym: SCN2B
• Protein Construction: A DNA sequence encoding the human SCN2B (O60939) (Met1-Ala159) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

SCN2B Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 30
• Molecular Mass: The recombinant human SCN2B /Fc is a disulfide-linked homodimer. The reduced monomer comprises 371 amino acids and has a predicted molecular mass of 42.2 kDa. The apparent molecular mass of the protein is approximately 53-57 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SCN2B protein

SCN2B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SCN2B Protein Description

SCN2B is essential for the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. Voltage-gated sodium channels (NaV) are composed of one pore-forming alpha-subunit, which may be associated with either one or more beta-subunits. Alpha-subunits are composed for four homologous domains, each of which contains six transmembrane segments. They are responsible for action potential initiation and propagation in excitable cells, including nerve, muscle, and neuroendocrine cell types. SCN2B interacts with TNR and may play a crucial role in clustering and regulation of activity of sodium channels at nodes of ranvier. SCN2B also causes an increase in the plasma membrane surface area and in its folding into microvilli.

References

1. Tan BH. et al., 2010, Heart Rhythm. 7 (6): 771-8.
2. Watanabe H. et al., 2009, Circ Arrhythm Electrophysiol. 2 (3): 268-75.