|Recombinant Human SCGN / Secretagogin Protein (Catalog#12411-H07E)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SCGN / Secretagogin(rh SCGN / Secretagogin; Catalog #12411-H07E; NP_008929.2; Asp 2-Pro 276). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
No cross-reactivity with E.coli cell lysate in ELISA
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human SCGN. The detection limit for Human SCGN is approximately 0.16 ng/well.
ELISA(Det): 0.1-1 µg/ml
This antibody will detect Human SCGN / Secretagogin in ELISA pair set (Catalog: # SEK12411). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 12411-MM02).
Secretagogin, also known as SCGN, is a secreted protein which is detectable in human serum after ischemic neuronal damage. It is a recently described calcium-binding protein. Secretagogin / SCGN is expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression of Secretagogin / SCGN is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. Secretagogin / SCGN is widely expressed in prostatic adenocarcinoma as opposed to adenocarcinomas in other organs. The function of Secretagogin / SCGN is unknown, but it has been suggested in beta-cells to influence calcium-influx and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. Secretagogin / SCGN is involved in the calcium metabolism of tumour cells and endothelial cells in a subset of neoplasms of the brain and its coverings. Secretagogin / SCGN is also a novel marker for neuroendocrine differentiation.