SCF / C-kit ligand Antibody (Antigen Affinity Purified)

SCF / C-kit ligand Antibody ( Antigen Affinity Purified ) Datasheet

SCF / C-kit ligand Antibody Product Information

• Immunogen: Recombinant Mouse SCF / C-kit ligand protein (Catalog#50487-M08B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse SCF / C-kit ligand (rM SCF / C-kit ligand; Catalog#50487-M08B; P20826-1; Met 1-Ala 189). SCF / C-kit ligand specific IgG was purified by Mouse SCF / C-kit ligand affinity chromatography.

SCF / C-kit ligand Antibody Usage Guide

• Specificity: Mouse SCF / C-kit ligand
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse KITL (aa 1-189) in WB. Using a DAB detection system, the detection limit for Mouse KITL (aa 1-189) is approximately 8 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse KITL (aa 1-189). The detection limit for Mouse KITL (aa 1-189) is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SCF / C-kit ligand Antibody Background

Kit ligand, also known as Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, Kitlg and KITL, is a single-pass type I membrane protein which belongs to theSCF family. KITL / kit ligand also belongs to the family of dimeric transmembrane growth factors. The soluble form of KIT ligand is a secreted protein. Mast cells are thought to participate in a variety of immune responses, such as parasite resistance and the allergic reaction. Mast cell development depends on stem cell factor (Kit ligand) and its receptor, c-Kit. KITL / kit ligand stimulates the proliferation of mast cells. KITL / kit ligand is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Efficient cell surface presentation of KITL / kit ligand is essential for the migration, proliferation, and survival of melanocytes, germ cells, hemopoietic stem cells, and mastocytes. KITL / kit ligand acts synergistically with other cytokines, probably interleukins. KITL / kit ligand plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. It exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes. KITL / kit ligand misexpression in some hyperpigmented lesions may open the avenue for Kitl-dependent treatment of pathological skin conditions.

References

1. Nishida,K. et al., 2002, Blood. 99 (5): 1866-9.
2. Paulhe,F. et al., 2004, J Biol Chem. 279 (53): 55545-55.
3. Fernandez,S.M. et al., 2008, Biol Reprod. 79 (2): 318-27.
4. Wehrle-Haller,B. et al., 2003, Pigment Cell Res.16 (3): 287-96.