Anti-SCF Antibody

Cat: 50487-RP02
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Anti-SCF Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SCF Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse SCF
Immunogen
Recombinant Mouse SCF / C-kit ligand protein (Catalog#50487-M08B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SCF / C-kit ligand (rM SCF / C-kit ligand; Catalog#50487-M08B; P20826-1; Met 1-Ala 189). SCF / C-kit ligand specific IgG was purified by Mouse SCF / C-kit ligand affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SCF Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SCF Antibody (Rabbit Polyclonal antibody) Images
Mouse KITLG/SCF/C-kit ligand Western blot (WB) 13677

Anti-KITL-His(1-189) rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:18 kDa

(We are unsure as to the identity of these extra bands.)

SCF Background Information

Similar to Kit ligand precursor (C-kit ligand) , also known as Stem cell factor (SCF), Mast cell growth factor (MGF) or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage- colony- stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.

Full Name
KIT ligand
References
  • McNiece IK, et al. (1995) Stem cell factor. J Leukoc Biol. 58(1): 14-22.
  • Besmer P, et al. (1993) The kit-ligand (steel factor) and its receptor c-kit/W: pleiotropic roles in gametogenesis and melanogenesis. Dev Suppl. 1993:125-37.
  • Mekori YA, et al. (1993) IL-3-dependent murine mast cells undergo apoptosis on removal of IL-3. Prevention of apoptosis by c-kit ligand. J Immunol. 151(7): 3775-84.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SCF Antibody Mouse Monoclonal ELISA 10451-MM23
    Anti-SCF Antibody Rabbit Polyclonal ELISA 10451-T16
    Anti-SCF Antibody Rabbit Monoclonal ELISA(Cap) 10451-R018
    Anti-SCF Antibody Rabbit Polyclonal ELISA 10451-T16
    Anti-SCF Antibody Rabbit Polyclonal ELISA 10451-RP01
    Anti-SCF Antibody Rabbit Polyclonal WB,ELISA 10451-T48
    Anti-SCF Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10451-R004
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