Human S1PR2 Gene cDNA Clone / ORF Clone

Human S1PR2 cDNA Clone Datasheet

S1PR2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004230.3
• cDNA Size: 1062
• Gene Synonym: EDG5; H218; LPB2; S1P2; AGR16; EDG-5; Gpcr13; S1PR2
• Source: Human

S1PR2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens sphingosine-1-phosphate receptor 2 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 255 C/A, not causing the amino acid variation.

S1PR2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

S1PR2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

S1PR2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCAGCT TGTACTCGGA GTACCTGAAC CCCAACAAGG TCCAGGAACA CTATAATTAT
61       ACCAAGGAGA CGCTGGAAAC GCAGGAGACG ACCTCCCGCC AGGTGGCCTC GGCCTTCATC
121      GTCATCCTCT GTTGCGCCAT TGTGGTGGAA AACCTTCTGG TGCTCATTGC GGTGGCCCGA
181      AACAGCAAGT TCCACTCGGC AATGTACCTG TTTCTGGGCA ACCTGGCCGC CTCCGATCTA
241      CTGGCAGGCG TGGCATTCGT AGCCAATACC TTGCTCTCTG GCTCTGTCAC GCTGAGGCTG
301      ACGCCTGTGC AGTGGTTTGC CCGGGAGGGC TCTGCCTTCA TCACGCTCTC GGCCTCTGTC
361      TTCAGCCTCC TGGCCATCGC CATTGAGCGC CACGTGGCCA TTGCCAAGGT CAAGCTGTAT
421      GGCAGCGACA AGAGCTGCCG CATGCTTCTG CTCATCGGGG CCTCGTGGCT CATCTCGCTG
481      GTCCTCGGTG GCCTGCCCAT CCTTGGCTGG AACTGCCTGG GCCACCTCGA GGCCTGCTCC
541      ACTGTCCTGC CTCTCTACGC CAAGCATTAT GTGCTGTGCG TGGTGACCAT CTTCTCCATC
601      ATCCTGTTGG CCATCGTGGC CCTGTACGTG CGCATCTACT GCGTGGTCCG CTCAAGCCAC
661      GCTGACATGG CCGCCCCGCA GACGCTAGCC CTGCTCAAGA CGGTCACCAT CGTGCTAGGC
721      GTCTTTATCG TCTGCTGGCT GCCCGCCTTC AGCATCCTCC TTCTGGACTA TGCCTGTCCC
781      GTCCACTCCT GCCCGATCCT CTACAAAGCC CACTACTTTT TCGCCGTCTC CACCCTGAAT
841      TCCCTGCTCA ACCCCGTCAT CTACACGTGG CGCAGCCGGG ACCTGCGGCG GGAGGTGCTT
901      CGGCCGCTGC AGTGCTGGAG GCCGGGGGTG GGGGTGCAAG GACGGAGGCG GGGCGGGACC
961      CCGGGCCACC ACCTCCTGCC ACTCCGCAGC TCCAGCTCCC TGGAGAGGGG CATGCACATG
1021     CCCACGTCAC CCACGTTTCT GGAGGGCAAC ACGGTGGTCT GA

 

Quality Control:cDNA sequencing results is available upon request.