Human S100B Gene cDNA Clone / ORF Clone

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10181-M	10µg ($95)		YES	Change Vector

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_006272.2

cDNA Size:   279

Synonym:   S100B, NEF, S100, S100beta

Source:  Human.

Description

cDNA Description:   ORF Clone of Homo sapiens S100 calcium binding protein B DNA

Vector:  Vector pMD18-T Simple

Sequence Description:   Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95℃ for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGTCTGAGC TGGAGAAGGC CATGGTGGCC CTCATCGACG TTTTCCACCA ATATTCTGGA
61       AGGGAGGGAG ACAAGCACAA GCTGAAGAAA TCCGAACTGA AGGAGCTCAT CAACAATGAG
121      CTTTCCCATT TCTTAGAGGA AATCAAAGAG CAGGAGGTTG TGGACAAAGT CATGGAAACA
181      CTGGACAATG ATGGAGACGG CGAATGTGAC TTCCAGGAAT TCATGGCCTT TGTTGCCATG
241      GTTACTACTG CCTGCCACGA GTTCTTTGAA CATGAGTGA

Quality Control: cDNA sequencing results is available upon request.

Product Catalog

Human S100B Gene cDNA Clone / ORF Clone