Mouse S100B Gene cDNA Clone / ORF Clone

S100B cDNA Clone Datasheet

S100B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009115.3
• cDNA Size: 279
• Gene Synonym: Bpb; AI850290; MGC74317; S100b
• Source: Mouse

S100B cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus S100 protein, beta polypeptide, neural DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

S100B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

S100B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

S100B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCGAGC TGGAGAAGGC CATGGTTGCC CTCATTGATG TCTTCCACCA GTACTCCGGG
61       CGAGAGGGTG ACAAGCACAA GCTGAAGAAG TCAGAACTGA AGGAGCTTAT CAACAACGAG
121      CTCTCTCACT TCCTGGAGGA AATCAAGGAG CAGGAAGTGG TGGACAAAGT GATGGAGACG
181      CTGGACGAAG ATGGGGATGG GGAGTGTGAC TTCCAGGAGT TCATGGCCTT CGTCGCCATG
241      GTGACCACGG CCTGCCATGA GTTCTTTGAA CATGAGTGA

 

Quality Control:cDNA sequencing results is available upon request.