S100B Protein

S100 Calcium-Binding Protein B Protein Datasheet

S100B Protein Price Information

500ug: Inquiring Price;      ≥1mg Bulk:  Inquiring Price

S100B Protein Product Information

• S100B Synonym: NEF, S100, S100beta
• Protein Construction: A DNA sequence encoding the human S100B (NP_006263.1) (Ser 2- Glu 92 ) was expressed with the fused Fc region of human IgG1 at the N-terminus.
• Source: Human
• Expression Host: Human Cells

S100B Protein QC Testing

• Purity: > 95%, as determined by SDS-PAGE
• Bio-activity: 1. Measured by its ability to bind mouse S100A1 in a functional ELISA.
  2. Measured by its ability to bind TP53 in a functional ELISA.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 20
• Molecular Mass: The recombinant human Fc/ S100B is a disulfide-linked homodimeric protein. The reduced monomer consists of 328 amino acids and has a predicted molecular mass of 37.2 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/S100B monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE:

S100B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100B Protein Description

S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. Although most of the S100 genes are located as a cluster at 1q21, S100B gene is located at 21q22.3. S100B is glial-specific and is synthesized in considerable amounts in the cytoplasm of astrocytes. This protein has been revealed to preform a variety of intracellular functions including cell proliferation, cell structure, energy metabolism, and calcium homeostasis. S100B is probably implicated in tumor progression by excessive down-regulation of the p53 tumor suppressor protein. As a neurotrophic factor, S100B stimulates neurite outgrowth and enhances survival of neurons during CNS development. Chromosomal rearrangements and abberant expression of S100B gene are associated with several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I  diabetes.

References

1. Allore, R. et al., 1988, Science. 239: 1311-1313.

2. Esposito,G. et al., 2008, J. Cell. Mol. Med. 12: 914-927.

3. Rothermundt, M. et al., 2003, Microsc. Res. Tech. 60: 614-632.

4. Lin, J. et al., 2004, J. Biol. Chem. 279: 34071-34077.

5. Nishiyama, H. et al., 2002, Proc. Natl. Acad. Sci. USA. 99: 4037-4042.