S100A9 / CAGB / p14 Protein (His Tag)

S100 Calcium-Binding Protein A9 Protein

S100A9 / CAGB / p14 Protein Product Information

• Synonym: 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14
• Protein Construction: A DNA sequence encoding the human S100A9 ( NP_002956.1 ) ( Met 1 - Pro 114 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

S100A9 / CAGB / p14 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: 1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA.
  2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA.
  3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED₅₀ for this effect is typically 10-30 μg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human S100A9 consists of 124 amino acids and predicts a molecular mass of 14.6 kDa. It migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE:

S100A9 / CAGB / p14 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A9 / CAGB / p14 Protein Description

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.
Human Protein S100-A9, also known as S100 calcium-binding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

References

1. Miyasaki, KT. et al., 1993, J. Dent. Res. 72: 517-23.
2. Fanò, G. et al., 1995, Prog Neurobiol. 46 (1): 71-82.
3. Vogl, T. et al., 2004, Blood. 104: 4260-8.
4. Viemann, D. et al., 2005, Blood. 105: 2955-62.
5. Nakatani, Y. et al., 2005, Mediators Inflamm. 2005: 280-292.
6. Bjoerk, P. et al., 2009, PLoS Biol. 7: E97-E97.