S100A9 Antibody

S100A9 Antibody Datasheet

S100A9 Antibody Product Information

• Immunogen: Recombinant Human S100A9 Protein (Catalog#11145-HNAE)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 018
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human S100A9 (rh S100A9; Catalog#11145-HNAE; NP_002956.1; Met 1 - Pro 114)

S100A9 Antibody Usage Guide

• Specificity: Human S100A9
• No cross-reactivity in ELISA with

  Human S100A13

  Human S100A1

  Human S100A2

  Human S100A7

  Human S100A8

  Mouse S100A6

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human S100A9 in WB.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human S100A9. The detection limit for Human S100A9 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A9 Antibody Background

Protein S100-A9, also known as S100 calcium-binding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

References

1. Miyasaki, KT. et al., 1993, J. Dent. Res. 72: 517-23.
2. Fanò, G. et al., 1995, Prog Neurobiol. 46 (1): 71-82.
3. Vogl, T. et al., 2004, Blood. 104: 4260-8. 
4. Viemann, D. et al., 2005, Blood. 105: 2955-62.
5. Nakatani, Y. et al., 2005, Mediators Inflamm. 2005:280-292.
6. Bjoerk, P. et al., 2009, PLoS Biol. 7: E97-E97.