Anti-S100A9 Antibody

Cat: 11145-RP02
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Anti-S100A9 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-S100A9 Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human S100A9
Immunogen
Recombinant Human S100A9 protein (Catalog#11145-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human S100A9 (rh S100A9; Catalog#11145-HNAE; NP_002956.1; Met 1-Pro 114). S100A9 specific IgG was purified by human S100A9 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-S100A9 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
IHC-P 1:2500-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-S100A9 Antibody (Rabbit Polyclonal antibody) Images
Human S100A9/MRP-14 Immunohistochemistry(IHC) 3643
Immunochemical staining of human S100A9 in human tonsil with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human S100A9/MRP-14 Immunohistochemistry(IHC) 3644
Immunochemical staining of human S100A9 in human spleen with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Anti-S100A9 Antibody Alternative Names
Anti-60B8AG Antibody;Anti-CAGB Antibody;Anti-CFAG Antibody;Anti-CGLB Antibody;Anti-L1AG Antibody;Anti-LIAG Antibody;Anti-MAC387 Antibody;Anti-MIF Antibody;Anti-MRP-14 Antibody;Anti-MRP14 Antibody;Anti-NIF Antibody;Anti-P14 Antibody
S100A9 Background Information

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calcium-binding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Full Name
S100 calcium binding protein A9
References
  • Miyasaki KT. et al., 1993, J Dent Res. 72: 517-23.
  • Fanò G. et al., 1995, Prog Neurobiol. 46 (1): 71-82.
  • Vogl T. et al., 2004, Blood. 104: 4260-8.
  • Viemann D. et al., 2005, Blood. 105: 2955-62.
  • Nakatani Y. et al., 2005, Mediators Inflamm. 2005: 280-92.
  • Bjoerk P. et al., 2009, PLoS Biol. 7: E97-E97.
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