S100A9 / CAGB Antibody (Antigen Affinity Purified)

Protein S100-A9 / S100A9 Antibody ( Antigen Affinity Purified )

S100A9 Antibody Product Information

• Immunogen: Recombinant human S100A9 protein ( Catalog#11145-HNAE )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, E.coli-derived, recombinant human S100A9 ( rh S100A9 ; Catalog#11145-HNAE ; NP_002956.1 ; Met 1 - Pro 114 ). S100A9 specific IgG was purified by human S100A9 affinity chromatography.

S100A9 Antibody Usage Guide

• Specificity: Human S100A9 / CAGB
• Immunochemistry: IHC-P: 0.05-2 μg/mL

  

  Immunochemical staining of human S100A9 in human spleen with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human S100A9 in human tonsil with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect S100A9 in WB. Using a DAB detection system, the detection limit for human S100A9 is approximately 4 ng/lane under non-reducing conditions and 16 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human S100A9. The detection limit for human S100A9 is 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A9 Antibody Background

Protein S100-A9, also known as S100 calcium-binding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

References

1. Miyasaki, KT. et al., 1993, J. Dent. Res. 72: 517-23.
2. Fanò, G. et al., 1995, Prog Neurobiol. 46 (1): 71-82.
3. Vogl, T. et al., 2004, Blood. 104: 4260-8. 
4. Viemann, D. et al., 2005, Blood. 105: 2955-62.
5. Nakatani, Y. et al., 2005, Mediators Inflamm. 2005:280-292.
6. Bjoerk, P. et al., 2009, PLoS Biol. 7: E97-E97.