S100A8 / CAGA / p8 Protein (His Tag)

S100 Calcium-Binding Protein A8 Protein

S100A8 / CAGA / p8 Protein Product Information

• Synonym: 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
• Protein Construction: A DNA sequence encoding the human S100A8 ( NP_002955.2 ) ( Met 1 - Glu 93 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

S100A8 / CAGA / p8 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Bio-activity: 1. Measured by its ability to bind recombinant human S100A9 in a functional ELISA.
  2. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED₅₀ for this effect is typically 3-15 μg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human S100A8 consists of 103 amino acids and predicts a molecular mass of 12.2 kDa. It migrates as an approximately 13 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: S100A8 protein

S100A8 / CAGA / p8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A8 / CAGA / p8 Protein Description

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.
Human Protein S100-A8, also known as S100 calcium-binding protein A8, Cystic fibrosis antigen, Migration inhibitory factor-related protein 8, S100A8, and CAGA, is a member of the S-100 family. S100A8 plays a role in various functions of myeloid cells by forming a heterocomplex with S100A9. S100A8 and S100A9 are known to be overexpressed in certain species of carcinomas. S100A8 plays an important role in dedifferentiation of thyroid carcinoma possibly by forming a complex with S100A9. S100A8 and S100A9 may also play a key role in inflammation-associated cancer.

References

1. Donato, R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-551.
2. Gebhardt, C. et al., 2006,  Biochem Pharmacol. 72 (11):1622-31.
3. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-1019.
4. Lim, SY. et al., 2008,  J Immunol. 181 (8): 5627-36.