S100A8 / CAGA Antibody (Antigen Affinity Purified)

S100 Calcium-Binding Protein A8 Antibody ( Antigen Affinity Purified )

S100A8 / CAGA Antibody Product Information

• Immunogen: Recombinant human S100A8 protein ( Catalog#11138-H07E )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human S100A8 ( rh S100A8 ; Catalog#11138-H07E ; NP_002955.2 ; Leu 2 - Glu 93 ). S100A8 specific IgG was purified by human S100A8 affinity chromatography

S100A8 / CAGA Antibody Usage Guide

• Specificity: Human S100A8 / CAGA
• Cross reactivity: Reacts with
  

  Mouse, Rat

• Immunochemistry: IHC-P: 0.05-2 μg/mL

  

  Immunochemical staining of human S100A8 in human spleen with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human S100A8 in human tonsil with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human S100A8 in WB. Using a DAB detection system, the detection limit for human S100A8 is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human S100A8. The detection limit for human S100A8 is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A8 / CAGA Antibody Background

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.

Human Protein S100-A8, also known as S100 calcium-binding protein A8, Cystic fibrosis antigen, Migration inhibitory factor-related protein 8, S100A8, and CAGA, is a member of the S-100 family. S100A8 plays a role in various functions of myeloid cells by forming a heterocomplex with S100A9. S100A8 and S100A9 are known to be overexpressed in certain species of carcinomas. S100A8 plays an important role in dedifferentiation of thyroid carcinoma possibly by forming a complex with S100A9. S100A8 and S100A9 may also play a key role in inflammation-associated cancer.

References

1. Donato, R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-551.
2. Gebhardt, C. et al., 2006,  Biochem Pharmacol. 72 (11):1622-31.
3. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-1019.
4. Lim, SY. et al., 2008,  J Immunol. 181 (8): 5627-36.