Mouse S100A7A Gene cDNA Clone / ORF Clone

S100A7A cDNA Clone Datasheet

S100A7A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_199422.1
• cDNA Size: 327
• Gene Synonym: Gm1020; S100A7f; S100a15; AY465109; S100A7L1; MGC130261; MGC130262; S100a17l1; S100a7a
• Source: Mouse

S100A7A cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus S100 calcium binding protein A7A DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

S100A7A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

S100A7A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

S100A7A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCAGACA CACCAGTGGA GGACTCCCTC TTCCAAATCA TACACTGCTT CCATCACTAT
61       GCTGCCCGGG AAGGGGACAA GGAGACCTTG TCCCTGGAGG AGTTGAAAGC TCTGCTCTTG
121      GATAGTGTGC CTCGCTTCAT GGACACCTTG GGCCGCAGGC AGCCATACTA CATCACAGAG
181      CTGTTCCGGG CAGCTGACAA AAACAAGGAC AACCAGATCT GCTTTGATGA GTTCCTGTAC
241      ATCTTGGGCA AGCTGGTGAA GGACTACCAT CTCCAGTTCC ACCGGCAGTT GTGTGCACAC
301      TACTGTACTG AGCACAGCCT CTACTAG

 

Quality Control:cDNA sequencing results is available upon request.