Mouse S100A6 Protein (Native)

S100 calcium binding protein A6 (calcyclin) Protein Datasheet

S100A6 / Calcyclin Protein Product Information

• S100A6 Synonym: 2A9, 5B10, CALCYCLIN, Cacy, PRA, S100a6
• Protein Construction: A DNA sequence encoding the mouse S100A6 (NP_035443.1) (Met 1-Lys 89 ) was expressed.
• Source: Mouse
• Expression Host: E.coli

S100A6 / Calcyclin Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse S100A6 consisting of 89 amino acids and has a calculated molecular mass of 10 kDa as estimated in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE:

S100A6 / Calcyclin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A6 / Calcyclin Protein Description

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium binding protein A6) is a member of the S100 family of proteins, and functions in stimulation of Ca2+-dependent insulin release, prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma. Mouse S100A6 is 99% and 96% aa identical to rat and human S100A6, respectively, and exits as monomer or disulfide-linked dimers.

References

1. Schäfer, B.W. et al., 1996, Trends Biochem. Sci. 21 (4): 134–140.
2. Donato,R. et al., 2003, Microsc. Res. Tech. 60 (6): 540–551.
3. Nowotny, M. et al., 2003, J. Biol. Chem. 278 (29): 26923-26928.
4. Marenholz, I. et al., 2004, Biochem. Biophys. Res. Commun. 322 (4): 1111–1122. 
5. Nonaka D, et al., 2008, J. Cutan. Pathol. 35 (11): 1014–1019.