|Recombinant Mouse S100A6 protein (Catalog#50229-MNAE)|
|0.2 μm filtered solution in PBS|
|This antibody was obtained from a rabbit immunized with purified, recombinant Mouse S100A6 / Calcyclin (rM S100A6; Catalog#50229-MNAE; Met 1-Lys 89; NP_035443.1).|
No cross-reactivity in ELISA with
Human S100A2 / CAN19 / S100L
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse S100A6. The detection limit for Mouse S100A6 is approximately 0.00245 ng/well.
ELISA(Cap): 0.5-4 µg/ml
This antibody will detect Mouse S100A6 in ELISA pair set (Catalog: # SEK50229). In a sandwich ELISA, it can be used as capture antibody.
IHC-P: 1-10 μg/mL
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium binding protein A6) is a member of the S100 family of proteins, and functions in prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma.