S100A6 / Calcyclin / CACY Antibody

Mouse S100A6 / Calcyclin / CACY Antibody

S100A6 / Calcyclin / CACY Antibody Product Information

• Immunogen: Recombinant mouse S100A6 protein ( Catalog#50229-MNAE )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 016
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse S100A6 / Calcyclin ( rM S100A6 ; Catalog#50229-MNAE ; Met 1 - Lys 89 ; NP_035443.1 ).

S100A6 / Calcyclin / CACY Antibody Usage Guide

• Specificity: Mouse S100A6 / Calcyclin / CACY
• No cross-reactivity in ELISA with

  Human S100A1

  Human S100A2 / CAN19 / S100L

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse S100A6 in WB. Using a DAB detection system, the detection limit for mouse S100A6 is approximately 0.25 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse S100A6. The detection limit for mouse S100A6 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A6 / Calcyclin / CACY Antibody Background

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium binding protein A6) is a member of the S100 family of proteins, and functions in stimulation of Ca2+-dependent insulin release, prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma. Mouse S100A6 is 99% and 96% aa identical to rat and human S100A6, respectively, and exits as monomer or disulfide-linked dimers

References

1. Schäfer, B.W. et al., 1996, Trends Biochem. Sci. 21 (4): 134-40.
2. Donato,R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-51.
3. Nowotny, M. et al., 2003, J. Biol. Chem. 278 (29): 26923-8.
4. Marenholz, I. et al., 2004, Biochem. Biophys. Res. Commun. 322 (4): 1111-22. 
5. Nonaka D, et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9.