Mouse S100A5 Protein (His Tag)

S100 calcium binding protein A5 Protein Datasheet

S100A5 Protein Product Information

• Synonym: S100D9
• Protein Construction: A DNA sequence encoding the mouse S100A5 (P63084) (Met 1-Lys 93) was expressed, with a polyhistide tag at the N-terminus.
• Source: Mouse
• Expression Host: E. Coli

S100A5 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse S100A5 consisting of 104 amino acids and has a calculated molecular mass of 12.3 kDa. rmS100A5 migrates as an approximately 13 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 20%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: S100A5 protein

S100A5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A5 Protein Description

S100 protein?is a family of low molecular weight protein found in vertebrates characterized by two?EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is?100%?soluble in ammonium sulfate?at neutral?pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.?? Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two?EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.

References

1. Sch?fer,B.W. et al., 2000, J Biol Chem. 275 (39):30623-30.
2. Gebhardt, C. et al., 2006, Biochem Pharmacol. 72 (11):1622-31.
3. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9.
4. Lim, SY. et al., 2008, J Immunol. 181 (8): 5627-36.
5. Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.