S100A5 Antibody (Antigen Affinity Purified)

S100A5 Antibody ( Antigen Affinity Purified ) Datasheet

S100A5 Antibody Product Information

• Immunogen: Recombinant Mouse S100A5 protein (Catalog#50427-M07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse S100A5 (rM S100A5; Catalog#50427-M07E; P63084; Met 1-Lys 93). S100A5 specific IgG was purified by Mouse S100A5 affinity chromatography.

S100A5 Antibody Usage Guide

• Specificity: Mouse S100A5
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse S100A5. The detection limit for Mouse S100A5 is less than 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A5 Antibody Background

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.

References

1. Schafer,B.W. et al., 2000, J Biol Chem. 275 (39):30623-30.
2. Gebhardt, C.et al., 2006, Biochem Pharmacol. 72 (11):1622-31.
3. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9.
4. Lim, SY. et al., 2008,J Immunol. 181 (8): 5627-36.
5. Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.