Mouse S100A3 Gene cDNA Clone / ORF Clone

S100A3 cDNA Clone Datasheet

S100A3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011310.2
• cDNA Size: 306
• Gene Synonym: S100E; S100a3
• Source: Mouse

S100A3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus S100 calcium binding protein A3 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 287 A/G resulting in the amino acid Glu substitution by Gly.

S100A3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

S100A3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

S100A3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCCGGC CCCTGGAGCA GGCAGTAGCT GCCATCGTGT GCACCTTCCA GGAGTATGCA
61       GGGCGCTGTG GGGATAAATA CAAGATCTGC CAGTCGGAGC TCAAGGAGTT GTTGCAGAAG
121      GAGCTGCCCA CCTGGACGCC GAGTGAGTTC CGGGAGTGTG ACTACAATAA ATTCATGAGT
181      GTTCTGGATA CCAACAAAGA CTGCGAAGTG GACTTTGGGG AGTACGTGCG CTCACTTGCC
241      AGCCTCTGTC TCTACTGCCA CGAGTACTTC AAAGAGTGCC CCCCTGGGCC TCCTTGCCCC
301      CAGTAG

 

Quality Control:cDNA sequencing results is available upon request.