S100A3 Antibody (Antigen Affinity Purified)

S100A3 Antibody ( Antigen Affinity Purified ) Datasheet

S100A3 Antibody Product Information

• Immunogen: Recombinant Human S100A3 protein (Catalog#11136-H10E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose

S100A3 Antibody Usage Guide

• Specificity: Human S100A3
• Immunochemistry: IHC-P: 0.5-2 μg/mL

  

  Immunochemical staining of human S100A3 in human tonsil with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing positive staining in tonsil crypt.

  Immunochemical staining of human S100A3 in human skinl with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing positive staining in hair follicle and sebaceous gland.

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human S100A3 in WB. Using a DAB detection system, the detection limit for Human S100A3 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human S100A3. The detection limit for Human S100A3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A3 Antibody Background

Protein S100-A3, also known as Protein S-100E, S100 calcium-binding protein A3, S100A3 and S100E, is a member of the S-100 family. S100A3 / S100E contains 2EF-hand domains. S100A3 / S100E is highly expressed in the differentiating cuticular cells within the hair follicle and organized into mature hair cuticles. High concentrations of S100A3 homotetramer might provide the millimolar level of Ca²⁺ required for hair cuticular barrier formation. S100A3 / S100E is a unique member of the Ca²⁺-binding S100 protein family with the highest cysteine content and affinity for Zn²⁺. S100A3 / S100E binds both calcium and zinc. S100A3 / S100E probably binds 2 zinc ions per molecule. It may be involved in calcium-dependent cuticle cell differentiation and hair shaft formation. S100A3 plays an important role in calcium-dependent processes leading to hair shaft formation. S100A3 / S100E is a unique protein among all members of the calcium-binding S100 family, is specifically expressed at the inner endocuticle of human hair fibers. Upon hair damage, S100A3 / S100E is released from hair fibers and possibly destabilizes the hair tissue architecture.

References

1. Kizawa, K. et al., 2008,J Biol Chem. 283 (8): 5004-13.
2. Kizawa, K. et al., 1998,J Invest Dermatol. 111 (5): 879-86.
3. Kizawa, K. et al., 2002, Biochem Biophys Res Commun. 299 (5): 857-62.
4. Fritz,G. et al., 2002, J Biol Chem. 277 (36): 33092-8.