S100A16 / S100F Protein (Native)

S100A16 Protein Datasheet

S100A16 Protein Product Information

• Synonym: AAG13, DT1P1A7, MGC17528, S100F
• Protein Construction: A DNA sequence encoding the human S100A16 ( Q96FQ6 ) ( Met 1 - Ser 103 ) was expressed and purified
• Source: Human
• Expression Host: E.coli

S100A16 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human S100A16 comprises 103 amino acids and has a predicted molecular mass of 11.9 kDa. It migrates as a 13 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Tris, pH 8.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: S100A16 protein

S100A16 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A16 Protein Description

S100A16, a EF-hand Ca(2+)-binding protein, is a new and unique member of the S100 protein super family. Its expression is ubiquitous and elevated in various types of tumors. S100 proteins are cell- and tissue-specific and are involved in many intra- and extracellular processes through interacting with specific target proteins. Differently from most S100 proteins, the conformational rearrangement upon calcium binding of S100A6 is minor. Its expression was found to be astrocyte-specific. The S100A16 protein was found to accumulate within nucleoli and to translocate to the cytoplasm in response to Ca(2+) stimulation. Calcium binding proteins of the S100 family play a central role in many intra- and extracellular processes and abnormal expression was observed in various tumors and human diseases. Immunoprecipitation analysis revealed that S100A16 could physically interact with tumor suppressor protein p53, also a known inhibitor of adipogenesis. Overexpression or RNA interference-initiated reduction of S100A16 led to the inhibition or activation of the expression of p53-responsive genes, respectively. S100A16 protein is a novel adipogenesis-promoting factor and that increased expression of S100A16 in 3T3-L1 adipocytes can have a negative impact on insulin sensitivity.

References

1. Marenholz I, et al., 2004, Biochem Biophys Res Commun. 313 (2): 237 - 44.
2. Babini E, et al., 2011, J Biol Inorg Chem. 16(2): 243-56.
3. Sturchler E, et al., 2006, J Biol Chem. 281(50): 38905-17.
4. Liu Y, et al., 2011, Endocrinology. 152(3): 903-11.