S100A16 Antibody (Antigen Affinity Purified)

S100A16 Antibody ( Antigen Affinity Purified ) Datasheet

S100A16 Antibody Product Information

• Immunogen: Recombinant Human S100A16 protein (Catalog#11137-HNAE)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human S100A16 (rh S100A16; Catalog#11137-HNAE; Q96FQ6; Met 1-Ser 103). S100A16 specific IgG was purified by Human S100A16 affinity chromatography.

S100A16 Antibody Usage Guide

• Specificity: Human S100A16
• Immunochemistry: IHC-P: 0.2-1 μg/mL

  

  Immunochemical staining of human S100A16 in human colon with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human S100A16 in human brain with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human S100A16. The detection limit for Human S100A16 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A16 Antibody Background

S100A16, a EF-hand Ca (2+)-binding protein, is a new and unique member of the S100 protein super family. Its expression is ubiquitous and elevated in various types of tumors. S100 proteins are cell- and tissue-specific and are involved in many intra- and extracellular processes through interacting with specific target proteins. Differently from most S100 proteins, the conformational rearrangement upon calcium binding of S100A6 is minor. Its expression was found to be astrocyte-specific. The S100A16 protein was found to accumulate within nucleoli and to translocate to the cytoplasm in response to Ca (2+) stimulation. Calcium binding proteins of the S100 family play a central role in many intra- and extracellular processes and abnormal expression was observed in various tumors and human diseases. Immunoprecipitation analysis revealed that S100A16 could physically interact with tumor suppressor protein p53, also a known inhibitor of adipogenesis. Overexpression or RNA interference-initiated reduction of S100A16 led to the inhibition or activation of the expression of p53-responsive genes, respectively. S100A16 protein is a novel adipogenesis-promoting factor and that increased expression of S100A16 in 3T3-L1 adipocytes can have a negative impact on insulin sensitivity.

References

1. Marenholz I, et al., 2004, Biochem Biophys Res Commun. 313 (2): 237-44.
2. Babini E, et al., 2011, J Biol Inorg Chem. 16 (2): 243-56.
3. Sturchler E, et al., 2006, J Biol Chem. 281 (50): 38905-17.
4. Liu Y, et al., 2011, Endocrinology. 152 (3): 903-11.