S100A14 / S114 Protein (His Tag)

Protein S100-A14 / S100 Calcium-Binding Protein A14 Protein

S100A14 / S114 Protein Product Information

• Synonym: BCMP84, S100A15
• Protein Construction: A DNA sequence encoding the native human S100A14 ( NP_065723.1 ) ( Gly 2 - His 104 ) was expressed, with a polyhistide tag at the N-terminus
• Source: Human
• Expression Host: E. coli

S100A14 / S114 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human S100A14 consisting of 114 amino acids and migrates as an 13 kDa band in SDS-PAGE under reducing conditions as predicted
• Formulation: Lyophilized from sterile 50mM Tris, 20% glycerol, pH 7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: S100A14 protein

S100A14 / S114 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A14 / S114 Protein Description

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. 
Protein S100-A14, also known as S100 calcium-binding protein A14, S114 and S100A14, is a cytoplasm protein which belongs to the S-100 family. It is expressed at highest levels in colon and at moderate levels in thymus, kidney, liver, small intestine, and lung. Low expression in heart and no expression is seen in brain, skeletal muscle, spleen, placenta and peripheral blood leukocytes.

References

1. Pietas A., et al., 2002, Genomics 79: 513-22.
2. Donato, R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-51.
3. Gebhardt, C. et al., 2006,  Biochem Pharmacol. 72 (11):1622-31.
4. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9.
5. Lim, SY. et al., 2008,  J Immunol. 181 (8): 5627-36.
6. Heibeck T.H. et al., 2009, J. Proteome Res. 8:3852-61.