S100A13 Antibody (Antigen Affinity Purified)

S100A13 Antibody ( Antigen Affinity Purified )

S100A13 Antibody Product Information

• Immunogen: Recombinant human S100A13 protein ( Catalog#10991-HNAE )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human S100A13 ( rh S100A13 ; Catalog#10991-HNAE ; NP_001019381.1 ; Met 1 - Lys 98 ). S100A13 specific IgG was purified by human S100A13 affinity chromatography

S100A13 Antibody Usage Guide

• Specificity: Human S100A13
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human S100A13 in WB. Using a DAB detection system, the detection limit for human S100A13 is approximately 1 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human S100A13. The detection limit for human S100A13 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A13 Antibody Background

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. 

Protein S100-A13, also known as S100 calcium-binding protein A13, is a member of the S-100 family. It contains two EF-hand domains. S100A13 binds two calcium ions per subunit and one copper ion. Binding of one copper ion does not interfere with calcium binding. S100A13 is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. S100A13 plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway.

References

1. Mandinova A. et al., 2003, J. Cell Sci. 116:2687-96.
2. Arnesano F. et al., 2005, Angew. Chem. Int. Ed. 44: 6341-4.
3. Viemann, D. et al., 2005, Blood. 105: 2955-62.
4. Nakatani, Y. et al., 2005, Mediators Inflamm. 2005: 280-92.
5. Bjoerk, P. et al., 2009, PLoS Biol. 7: E97-E97.