S100A12 / CAGC Antibody (Antigen Affinity Purified)

S100 Calcium-binding Protein A12 Antibody ( Antigen Affinity Purified )

S100A12 / CAGC Antibody Product Information

• Immunogen: Recombinant human S100A12 protein ( Catalog#11143-HNAE )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human S100A12 ( rh S100A12 ; Catalog#11143-HNAE ; NP_005612.1 ; Met 1 - Glu 92 ). S100A12 specific IgG was purified by human S100A12 affinity chromatography

S100A12 / CAGC Antibody Usage Guide

• Specificity: Human S100A12 / CAGC
• Cross reactivity: Does not react with
  

  Mouse, Rat

• Immunochemistry: IHC-P: 0.1-1 μg/mL

  

  Immunochemical staining of human S100A12 in human spleen with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human S100A12 in WB. Using a DAB detection system, the detection limit for human S100A12 is approximately 2 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human S100A12. The detection limit for human S100A12 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A12 / CAGC Antibody Background

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. 

Protein S100-A12, also known as S100 calcium-binding protein A12, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, and S100A12, is a member of the S-101 family. Like the majority of S100 proteins, S100A12 is a dimer, with the interface between the two subunits being composed mostly of hydrophobic residues. The fold of S100A12 is similar to the other known crystal and solution structures of S100 proteins, except for the linker region between the two EF-hand motifs. S100A12 plays an important role in the inflammatory response.

References

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2. Foell, D. et al., 2003, Lancet  361 (9365):1270-2.
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5. Nakatani, Y. et al., 2005, Mediators Inflamm. 2005: 280-292.
6. Bjoerk, P. et al., 2009, PLoS Biol. 7: E97-E97.