Mouse S100A1 Protein (His Tag)

S100 calcium binding protein A1 Protein Datasheet

S100A1 Protein Product Information

• Synonym: AI266795, S100, S100a
• Protein Construction: A DNA sequence encoding the mouse S100A1 (P56565) (Gly 2-Ser 94) was expressed, with a polyhistide tag at the N-terminus.
• Source: Mouse
• Expression Host: E.coli

S100A1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to bind human AGER in a functional ELISA.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse S100A1 consisting of 109 amino acids and has a calculated molecular mass of 12.5 kDa. rmS100A1 migrates as an approximately 14KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: S100A1 protein

S100A1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

S100A1 Protein Description

S100A1 is a Ca²⁺ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca²⁺-induced Ca²⁺ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation.. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

References

1. Remppis A .et al., 1996, Biochim Biophs Acta. 1313: 253-257.
2. Most P. et al., 2001, Proc Natl Acad Sci. 98: 13889-13894.
3. Okada M. et al., 2004, J Biol Chem. 279: 4221-4233.
4. Schafer WE. et al., 1995, Genomics. 25: 638-643.
5. Landar A. et al., 1996, Cell Calcium. 20: 279-285.