S100A1 Antibody (Antigen Affinity Purified)

S100A1 Antibody ( Antigen Affinity Purified ) Datasheet

S100A1 Antibody Product Information

• Immunogen: Recombinant Mouse S100A1 protein (Catalog#50266-M07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse S100A1 (rM S100A1; Catalog#50266-M07E; P56565; Gly 2-Ser 94). S100A1 specific IgG was purified by Mouse S100A1 affinity chromatography.

S100A1 Antibody Usage Guide

• Specificity: Mouse S100A1
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of mouse S100A1 in mouse uterus with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.

  Immunochemical staining of mouse S100A1 in mouse brain with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).

  

  Immunochemical staining of mouse S100A1 in mouse heart with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to cardiac muscle.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse S100A1. The detection limit for Mouse S100A1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

S100A1 Antibody Background

S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation.. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

References

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2. Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894.
3. Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233.
4. Schafer, W.E. et al., 1995, Genomics. 25: 638-643.
5. Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.