Mouse Rspo2 Gene cDNA Clone / ORF Clone

Rspo2 cDNA Clone Datasheet

Rspo2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_172815.3
• cDNA Size: 732
• Gene Synonym: ftls; AA673245
• Source: Mouse

Rspo2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus R-spondin 2 homolog DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Rspo2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Rspo2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

Rspo2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGTTTTT GCCTCTTCTC ATTTGCCCTC ATCATTCTGA ACTGTATGGA TTACAGCCAG
61       TGCCAAGGCA ACCGATGGAG ACGCAATAAG CGAGCTAGTT ATGTATCAAA TCCCATTTGC
121      AAGGGTTGTT TGTCTTGTTC GAAGGACAAT GGTTGCAGCC GATGTCAACA GAAGTTGTTC
181      TTTTTCCTTC GAAGAGAAGG AATGCGTCAG TATGGAGAGT GCCTGCATTC CTGCCCATCA
241      GGGTATTATG GACACCGAGC CCCAGATATG AACAGATGTG CACGATGCAG AATAGAAAAC
301      TGTGATTCTT GCTTTAGCAA AGACTTTTGT ACGAAGTGCA AAGTAGGCTT TTATTTGCAT
361      AGAGGCCGCT GCTTTGATGA ATGTCCAGAT GGTTTTGCAC CGTTAGATGA GACTATGGAA
421      TGTGTAGAAG GTTGTGAAGT TGGTCATTGG AGCGAATGGG GAACGTGTAG CAGAAACAAC
481      CGCACGTGTG GATTTAAATG GGGTCTGGAA ACCAGAACAC GGCAGATTGT TAAAAAGCCA
541      GCAAAAGACA CAATACCATG TCCGACCATT GCGGAGTCCA GGAGATGCAA GATGGCCATG
601      AGGCACTGTC CAGGAGGAAA GAGAACACCA AAGGCAAAAG AGAAGAGAAA CAAGAAGAAG
661      AGGCGGAAGC TGATTGAGAG AGCCCAAGAG CAGCACAGCG TCTTCCTCGC TACAGACAGA
721      GTGAACCAAT AA

 

Quality Control:cDNA sequencing results is available upon request.