Anti-RhoA Antibody

Cat: 12441-RP02
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Anti-RhoA Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-RhoA Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human RhoA
Immunogen
Recombinant Human RhoA protein (Catalog#12441-H07B)
Preparation
Produced in rabbits immunized with purified, recombinant Human RhoA (rh RhoA; Catalog#12441-H07B; P61586-1; Met 1-Leu 193). RhoA specific IgG was purified by Human RhoA affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-RhoA Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-RhoA Antibody (Rabbit Polyclonal antibody) Images
Human RhoA Immunoprecipitation(IP) 8138

RHOA was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-RHOA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-RHOA rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 21 kDa

Observed band size: 21 kDa

Human RhoA Western blot (WB) 19020

Anti-RHOA rabbit polyclonal antibody at 1:500 dilution

Lane A: HL60 Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lane D: SKBR3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:18 kDa

Anti-RhoA Antibody Alternative Names
Anti-ARH12 Antibody;Anti-ARHA Antibody;Anti-RHO12 Antibody;Anti-RHOH12 Antibody
RhoA Background Information

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

Full Name
ras homolog family member A
References
  • Kiss C, et al.,1997, Cytogenet. Cell Genet. 79 (3-4): 228-30.
  • Anastasiadis,P.Z. et al., 2000, Nat Cell Biol. 2 (9):637-44.
  • Yiu G, et al.,2006, Nat. Rev. Neurosci. 7 (8): 617-27.
  • Wang,HR. et al., 2006, Methods Enzymol. 406 :437-47.
  • Heo,J. et al., 2006, Biochemistry. 45 (48):14481-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-RhoA Antibody (FITC) Rabbit Monoclonal FCM 12441-R036-F
    Anti-RhoA Antibody Rabbit Monoclonal ELISA 12441-R101
    Anti-RhoA Antibody (FITC) Mouse Monoclonal FCM 12441-MM03-F
    Anti-RhoA Antibody (PE) Mouse Monoclonal FCM 12441-MM03-P
    Anti-RhoA Antibody Mouse Monoclonal ELISA 12441-MM09
    Anti-RhoA Antibody Rabbit Monoclonal FCM 12441-R036
    Anti-RhoA Antibody Mouse Monoclonal ELISA,FCM,ICC/IF,IF 12441-MM03
    Anti-RhoA Antibody Rabbit Monoclonal WB,IP 12441-R103
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