|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
RHOA was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate2 µL anti-RHOA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-RHOA rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 21 kDa
Observed band size: 21 kDa
Anti-RHOA rabbit polyclonal antibody at 1:500 dilution
Lane A: HL60 Whole Cell Lysate
Lane B: MCF7 Whole Cell Lysate
Lane C: HeLa Whole Cell Lysate
Lane D: SKBR3 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:22 kDa
Observed band size:18 kDa
Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-RhoA Antibody (FITC)||Rabbit||Monoclonal||FCM||12441-R036-F|
|Anti-RhoA Antibody (FITC)||Mouse||Monoclonal||FCM||12441-MM03-F|
|Anti-RhoA Antibody (PE)||Mouse||Monoclonal||FCM||12441-MM03-P|