_×
RhoA Antibody (FITC), Mouse MAb
  0 products, total $0.+86-400-890-9989      Login |  Register 中文한국어

Browse By Molecule:

Your Position: Home > Antibody > Mouse MAb Antibody > RhoA Antibody (FITC), Mouse MAb

RhoA Antibody (FITC), Mouse MAb PDF Download

Catalog   Size (Price) Quantity In Stock Operation
12441-MM03-F  
YES   
 

Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

RhoA Antibody Datasheet

  Order or Inquire for RhoA Antibody product Quality antibodies Antibody production services
 

RhoA Antibody Product Information

Immunogen :

Recombinant Human RhoA protein (Catalog#12441-H07B)

Reagents : FITC-conjugated mouse monoclonal antibody

Clone ID :

03

Ig Type :

Mouse IgG2a

Concentration :

10 μl/Test, 0.1 mg/ml

Formulation : Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation :

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; Catalog#12441-H07B; P61586-1; Met 1-Leu 193) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

RhoA Antibody Usage Guide

Specificity :

Human RhoA

Flow Cytometry :
RhoA Flow Cytometry

Profile of anti-RHOA reactivity on HeLa cells analyzed by flow cytometry. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with FITC conjugated Mouse anti-RHOA (12441-MM03-F), The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Flow cytometry was performed on a BD FACSCalibur flow cytometry system

Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

Storage : This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

RhoA Antibody Related Products & Topics

RhoA Antibody Related Areas:

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
RhoA Human RhoA Protein, Recombinant 12441-H07B

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human RhoA ELISA, FCM RhoA Antibody 12441-MM03
Human RhoA FCM RhoA Antibody ( FITC ) 12441-MM03-F
Human RhoA WB, ELISA RhoA Antibody 12441-MM09
Human RhoA WB, ELISA RhoA Antibody (Antigen Affinity Purified) 12441-RP02

RhoA Antibody Background

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to thesmall GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

References

  1. Kiss C, et al.,1997, Cytogenet. Cell Genet. 79 (3-4): 228-30.
  2. Anastasiadis,P.Z. et al., 2000, Nat Cell Biol. 2 (9): 637-44.
  3. Yiu G, et al.,2006,Nat. Rev. Neurosci. 7 (8): 617-27.
  4. Wang,HR. et al., 2006, Methods Enzymol. 406: 437-47.
  5. Heo,J. et al.,2006, Biochemistry. 45 (48): 14481-9.
 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"