RhoA Antibody (FITC), Mouse MAb
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RhoA Antibody (FITC), Mouse MAb PDF Download

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Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

RhoA Antibody Datasheet

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RhoA Antibody Product Information

Immunogen :

Recombinant Human RhoA protein (Catalog#12441-H07B)

Reagents : FITC-conjugated mouse monoclonal antibody

Clone ID :


Ig Type :

Mouse IgG2a

Concentration :

10 μl/Test, 0.1 mg/ml

Formulation : Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation :

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; Catalog#12441-H07B; P61586-1; Met 1-Leu 193) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

RhoA Antibody Usage Guide

Specificity :

Human RhoA

Flow Cytometry :
RhoA Flow Cytometry

Profile of anti-RHOA reactivity on HeLa cells analyzed by flow cytometry. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with FITC conjugated Mouse anti-RHOA (12441-MM03-F), The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Flow cytometry was performed on a BD FACSCalibur flow cytometry system

Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

Storage : This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

RhoA Antibody Related Products & Topics

RhoA Antibody Related Areas:


Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
RhoA Human RhoA Protein, Recombinant 12441-H07B


Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human RhoA ELISA, FCM RhoA Antibody 12441-MM03
Human RhoA FCM RhoA Antibody ( FITC ) 12441-MM03-F
Human RhoA WB, ELISA RhoA Antibody 12441-MM09
Human RhoA WB, ELISA RhoA Antibody (Antigen Affinity Purified) 12441-RP02

RhoA Antibody Background

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to thesmall GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.


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