RhoA Antibody ( FITC )

RhoA Antibody Datasheet

RhoA Antibody Product Information

• Immunogen: Recombinant Human RhoA protein (Catalog#12441-H07B)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 03
• Ig Type: Mouse IgG2a
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; Catalog#12441-H07B; P61586-1; Met 1-Leu 193) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

RhoA Antibody Usage Guide

• Specificity: Human RhoA
• Flow Cytometry:
  

  Profile of anti-RHOA reactivity on HeLa cells analyzed by flow cytometry. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with FITC conjugated Mouse anti-RHOA (12441-MM03-F), The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

RhoA Antibody Background

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to thesmall GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

References

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2. Anastasiadis,P.Z. et al., 2000, Nat Cell Biol. 2 (9): 637-44.
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4. Wang,HR. et al., 2006, Methods Enzymol. 406: 437-47.
5. Heo,J. et al.,2006, Biochemistry. 45 (48): 14481-9.