- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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RhoA Antibody Datasheet
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RhoA Antibody Product Information
Recombinant Human RhoA protein (Catalog#12441-H07B)
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; Catalog#12441-H07B; P61586-1; Met 1-Leu 193).
RhoA Antibody Usage Guide
|Flow Cytometry :||
Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-RHOA (12441-MM03), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow cytometry was performed on a BD FACSCalibur flow cytometry system
Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
ICC/IF: 10-25 μg/mL
|Direct ELISA :||This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human RHOA.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
RhoA Antibody Related Products & Topics
RhoA Antibody Related Areas:
RhoA Antibody Background
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- Anastasiadis,P.Z. et al., 2000, Nat Cell Biol. 2 (9): 637-44.
- Yiu G, et al.,2006,Nat. Rev. Neurosci. 7 (8): 617-27.
- Wang,HR. et al., 2006, Methods Enzymol. 406: 437-47.
- Heo,J. et al.,2006, Biochemistry. 45 (48): 14481-9.