Mouse Resistin / ADSF / RETN Protein

Resistin Protein Datasheet

Resistin / ADSF / RETN Protein Product Information

• Synonym: ADSF, Fizz3, Rstn, Xcp4
• Protein Construction: A DNA sequence encoding the mouse RETN (Q99P87) (Ser 21-Ser 114) was fused with the Fc region of human IgG₁ at the N-terminus.
• Source: Mouse
• Expression Host: Human Cells

Resistin / ADSF / RETN Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant mouse RETN/Fc is a disulfide-linked homodimer. The reduced monomer comprises 354 amino acids and has a calculated molecular mass of 38.6 kDa. The apparent molecular mass of the monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Resistin / ADSF / RETN protein

Resistin / ADSF / RETN Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Resistin / ADSF / RETN Protein Description

Resistin also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) or RETN is a cysteine-rich protein belonging to the resistin / FIZZ family. Resistin is a hormone produced by fat cells and is a postulated to be a molecular link between obesity and type 2 diabetes. This hormone is expressed only in fatty tissues and seems to suppress insulin ability to stimulate glucose uptake into adipose cells. RETNα is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue. Another family member, RETNβ, is a secreted protein expressed only in the gastrointestinal tract, particularly the colon, in both mouse and human. Plasma resistin levels are correlated with markers of inflammation and are predictive of coronary atherosclerosis in humans, independent of CRP.

References

1. Verma S. et al., 2003, Circulation. 108 (6): 736-40.
2. Reilly MP. et al., 2005, Circulation. 111 (7): 932-9.
3. Steppan CM. et al., 2002, Trends Endocrinol Metab. 13 (1): 18-23.