Mouse REG3A Protein (His Tag)

Regenerating islet-derived 3 Alpha Protein Datasheet

Reg3a Protein Product Information

• Reg3a Synonym: AV063448,Rega3a
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Thr 457) of mouse REG3A (NP_035389.1) precursor was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

Reg3a Protein QC Testing

• Purity: > 95%, as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Thr 22
• Molecular Mass: The secreted recombinant mouse REG3A consists of 161 amino acids and has a calculated molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE:

Reg3a Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Reg3a Protein Description

Regenerating islet-derived 3 alpha, also known as REG3A, hepatocarcinoma intestine pancreas (HIP), pancreatitis associated protein (PAP), is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It is a member of the regeneration (REG) family and has similarity to the C-type lectin superfamily. REG3A is mainly expressed in the intestine, with lower expression detected in healthy pancreas. It is also detected in pancreatic juice as a result of pancreatic inflammation. The enhanced expression of REG3A gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for REG3A gene but the full length nature of some transcripts is not yet known. The regeneration (REG) family consists of secretory proteins involved in liver, pancreatic, gastric and intestinal cell proliferation or differentiation. Members of the REG family are divided into four subclasses, designated types I, II, III and IV. They have been implicated in the regulation of cell growth, tumorigenesis and the progression of cancer.

References

1. Vassar, R. et al., 1999, Science. 286: 735-741.
2. Yan, R. et al., 1999, Nature. 402: 533-537.
3. Luo, Y. et al., 2001, Nat. Neurosci. 4:231-232.