Recombinant Protein

Recombinant Proteins Overview

Sino Biological provides a wide selection of quality recombinant proteins for research, which are directed towards:

• Adhesion molecules
• Cytokines
• Chemokines
• Growth factors
• Kinases
• Proteases
• Phosphatases
• Transcription factors
• Channel proteins
• Other

Research Topics:

• Over 400 proteins for cancer research
• Over 550 proteins related to immunology
• Over 220 proteins related to neuroscience
• Over 200 proteins for stem cell research
• Over 300 enzymatic proteins
• Over 70 viral proteins

Proteins developed as biomarkers and disease therapeutic targets.

Species:

• Human
• Mouse
• Rat
• Rhesus
• Canine
• Bovine
• Influenza Virus
• HIV (Human Immunodeficiency Virus)
• HCMV (Human Cytomegalovirus)
• RSV (Human Respiratory Syncytial Virus)

Influenza Viral Proteins:

Over 60 influenza viral proteins, including influenza hemagglutinin (HA), influenza neuraminidase (NA), and influenza nucleoprotein (NP), from 9 influenza subtypes and 28 influenza strains.

• Influenza virus research tools
• H1N1 (HA, NA, NP)
• H5N1 (HA, NA)
• H9N2, H1N3, H2N2, H3N2, H7N7, H10N3, influenza B

Our Proteins Feature:

Expression Host and Bioactivity

• Over 90% recombinant proteins are human cell-expressed.Human cell expression ensures the proper folding, glycosylation, gamma-carboxylation and other post-translational modification of recombinant proteins.
• The bioactivity assay results of several recombinant protein products are shown in table 1.

Table 1 Protein Bioactivity

Protein name	Sino Biological	US leading brand
	Bioactivity (ED50 ng/ml)
GCSFR	1-4	1000-4000
NTRK1	40-150	800-4000
bFGF	0.02-0.1	0.1-0.6
EGF	0.047	0.02-0.1
NGF	0.4	0.2-2
aFGF	0.05-0.2	0.1-0.3
FGFR4	2-6	2-6
GCSF-beta	0.022	0.01-0.06
TGF-beta 1	0.04-0.2	0.04-0.2
PDGF	124.5	70-350
	Bioactivity (IC50)
SerpinA1	< 3.0 nM	< 5.0 nM
Serpin F2	< 0.5 nM	< 2.0 nM
Mouse Serpin F2	< 0.5 nM	< 1.0 nM
Mouse TFPI	< 0.15 nM	< 1.0 nM
TFPI	< 0.4 nM	< 0.75 nM
TFPI2	< 2.0 nM	< 5.0 nM

Purity

• The majority of our recombinant proteins are purified to above 95% purity, suitable for most in vitro experiments.
• Some recombinant proteins are purified to purity over 99%.
• The SDS-PAGE images of recombinant proteins are provided.

Endotoxin Level

• Human cell-expression ensures lower endotoxin level of most recombinant proteins.
• Recombinant proteins were assessed for endotoxin using the Limulus Amoebocyte Lysates (LAL) assay, and the endotoxin levels are lower than 1.0 EU / μg.
• The endotoxin level of TGF-beta 1 (CATALOG #: 10804-HNAH) is 0.15 EU / mg protein, and bFGF (CATALOG #: 10014-HNAE) is < 1.0 EU per mg protein as determined by the LAL method.

Formulation and Stability

• All recombinant proteins are carrier-free. The addition of a carrier protein (such as BSA) to protein solutions may disturb your experiments.
• Most recombinant proteins are lyophilized from a sterile filtered solution containing protective agent: trehalose and mannitol. Trehalose is effective for stabilizing protein when lyophilized and during storage in solid state, and also for maximizing recovery upon reconstitution. Mannitol effectively prevents aggregation of protein.

Affordable Price

• Dramatic cost saving for quality recombinant proteins.

Bulk Quantities

• Big discount for bulk order.
• Please contact us for a bulk order quote.

New Protein Update

• Over 30 protein products are produced per week.
• New product information is updated weekly.