|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat UCHL3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80087-ACG|
|Rat UCHL3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80087-ACR|
|Rat UCHL3 ORF mammalian expression plasmid, N-GFPSpark tag||RG80087-ANG|
|Rat UCHL3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80087-ANR|
|Rat UCHL3 ORF mammalian expression plasmid, C-Flag tag||RG80087-CF|
|Rat UCHL3 ORF mammalian expression plasmid, C-His tag||RG80087-CH|
|Rat UCHL3 ORF mammalian expression plasmid, C-Myc tag||RG80087-CM|
|Rat UCHL3 ORF mammalian expression plasmid, C-HA tag||RG80087-CY|
|Rat UCHL3 Gene cDNA clone plasmid||RG80087-G|
|Rat UCHL3 ORF mammalian expression plasmid, N-Flag tag||RG80087-NF|
|Rat UCHL3 ORF mammalian expression plasmid, N-His tag||RG80087-NH|
|Rat UCHL3 ORF mammalian expression plasmid, N-Myc tag||RG80087-NM|
|Rat UCHL3 ORF mammalian expression plasmid, N-HA tag||RG80087-NY|
|Rat UCHL3 natural ORF mammalian expression plasmid||RG80087-UT|
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Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.