|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TNFSF14 ORF mammalian expression plasmid, C-GFPSpark tag||RG80175-ACG|
|Rat TNFSF14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80175-ACR|
|Rat TNFSF14 ORF mammalian expression plasmid, C-Flag tag||RG80175-CF|
|Rat TNFSF14 ORF mammalian expression plasmid, C-His tag||RG80175-CH|
|Rat TNFSF14 ORF mammalian expression plasmid, C-Myc tag||RG80175-CM|
|Rat TNFSF14 ORF mammalian expression plasmid, C-HA tag||RG80175-CY|
|Rat TNFSF14 Gene cDNA clone plasmid||RG80175-G|
|Rat TNFSF14 ORF mammalian expression plasmid, N-Flag tag||RG80175-NF|
|Rat TNFSF14 ORF mammalian expression plasmid, N-His tag||RG80175-NH|
|Rat TNFSF14 ORF mammalian expression plasmid, N-Myc tag||RG80175-NM|
|Rat TNFSF14 ORF mammalian expression plasmid, N-HA tag||RG80175-NY|
|Rat TNFSF14 natural ORF mammalian expression plasmid||RG80175-UT|
|Learn more about expression Vectors|
LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble nonsignaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+ and CD8+ T cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.