|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TNFRSF13B ORF mammalian expression plasmid, C-GFPSpark tag||RG80157-ACG|
|Rat TNFRSF13B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80157-ACR|
|Rat TNFRSF13B ORF mammalian expression plasmid, C-Flag tag||RG80157-CF|
|Rat TNFRSF13B ORF mammalian expression plasmid, C-His tag||RG80157-CH|
|Rat TNFRSF13B ORF mammalian expression plasmid, C-Myc tag||RG80157-CM|
|Rat TNFRSF13B ORF mammalian expression plasmid, C-HA tag||RG80157-CY|
|Rat TNFRSF13B Gene cDNA clone plasmid||RG80157-G|
|Rat TNFRSF13B ORF mammalian expression plasmid, N-Flag tag||RG80157-NF|
|Rat TNFRSF13B ORF mammalian expression plasmid, N-His tag||RG80157-NH|
|Rat TNFRSF13B ORF mammalian expression plasmid, N-Myc tag||RG80157-NM|
|Rat TNFRSF13B ORF mammalian expression plasmid, N-HA tag||RG80157-NY|
|Rat TNFRSF13B natural ORF mammalian expression plasmid||RG80157-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily, member 13B (TNFRSF13B) also known as Transmembrane activator and CAML interactor (TACI) and CD267 antigen, is a member of the tumor necrosis factor receptor superfamily. TNFRSF13B is a trimeric cytokine receptor that binds tumor necrosis factors (TNF). The receptor cooperates with an adaptor protein which is important in determining the outcome of the response. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors or tumour necrosis factor (TNFR), on their surface. Tumour necrosis factors (TNFR) detect the presence of extracellular death signals and, in response, they rapidly ignite the cell's intrinsic apoptosis machinery. TACI/TNFRSF13B/CD267 induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand.