|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat TLR4 ORF mammalian expression plasmid, C-GFPSpark tag||RG80389-ACG|
|Rat TLR4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80389-ACR|
|Rat TLR4 ORF mammalian expression plasmid, C-Flag tag||RG80389-CF|
|Rat TLR4 ORF mammalian expression plasmid, C-His tag||RG80389-CH|
|Rat TLR4 ORF mammalian expression plasmid, C-Myc tag||RG80389-CM|
|Rat TLR4 ORF mammalian expression plasmid, C-HA tag||RG80389-CY|
|Rat TLR4 Gene cDNA clone plasmid||RG80389-G|
|Rat TLR4 ORF mammalian expression plasmid, N-Flag tag||RG80389-NF|
|Rat TLR4 ORF mammalian expression plasmid, N-His tag||RG80389-NH|
|Rat TLR4 ORF mammalian expression plasmid, N-Myc tag||RG80389-NM|
|Rat TLR4 ORF mammalian expression plasmid, N-HA tag||RG80389-NY|
|Rat TLR4 natural ORF mammalian expression plasmid||RG80389-UT|
|Learn more about expression Vectors|
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).