|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat TLR4 ORF mammalian expression plasmid, C-GFPSpark tag||RG80389-ACG|
|Rat TLR4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80389-ACR|
|Rat TLR4 ORF mammalian expression plasmid, C-Flag tag||RG80389-CF|
|Rat TLR4 ORF mammalian expression plasmid, C-His tag||RG80389-CH|
|Rat TLR4 ORF mammalian expression plasmid, C-Myc tag||RG80389-CM|
|Rat TLR4 ORF mammalian expression plasmid, C-HA tag||RG80389-CY|
|Rat TLR4 Gene cDNA clone plasmid||RG80389-G|
|Rat TLR4 ORF mammalian expression plasmid, N-Flag tag||RG80389-NF|
|Rat TLR4 ORF mammalian expression plasmid, N-His tag||RG80389-NH|
|Rat TLR4 ORF mammalian expression plasmid, N-Myc tag||RG80389-NM|
|Rat TLR4 ORF mammalian expression plasmid, N-HA tag||RG80389-NY|
|Rat TLR4 natural ORF mammalian expression plasmid||RG80389-UT|
|Learn more about expression Vectors|
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).