CD43 cDNA ORF Clone, Rat, N-His tag

Cat: RG80432-NH
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CD43 Reagents
CRO Services
Product recommended

All CD43 Reagents
CD43 cDNA ORF Clone, Rat, N-His tag General Information
Gene
Species
Rat
NCBI Ref Seq
RefSeq ORF Size
1173 bp
Description
Full length Clone DNA of Rat persephin with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-SP-N-His
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD43 Background Information

CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals; it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. In addition, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.

Full Name
sialophorin
References
  • . Layseca-Espinosa E, et al. (2003) Journal of Leukocyte Biology. 74(6): 1083-93.
  • Cannon JL, et al. (2011) Mol Biol Cell. 22(7):954-63.
  • Pallant A,et al. (1989). Proc Natl Acad Sci. 86 (4): 1328–32.
  • CD43 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CD43 cDNA ORF Clone in Cloning Vector, Rat RG80432-G
    CD43 cDNA ORF Clone, Rat, untagged RG80432-UT
    CD43 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80432-ACGLN
    CD43 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80432-ACG
    CD43 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80432-ACR
    CD43 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80432-CF
    CD43 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80432-NF
    CD43 cDNA ORF Clone, Rat, C-His tag RG80432-CH
    CD43 cDNA ORF Clone, Rat, C-HA tag RG80432-CY
    CD43 cDNA ORF Clone, Rat, N-HA tag RG80432-NY
    CD43 cDNA ORF Clone, Rat, C-Myc tag RG80432-CM
    CD43 cDNA ORF Clone, Rat, N-Myc tag RG80432-NM
    CD43 qPCR Primer Pairs, Rat RP300405

    CD43 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD43 cDNA ORF Clone in Cloning Vector, Mouse MG50735-G
    CD43 cDNA ORF Clone, Mouse, untagged MG50735-UT
    CD43 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50735-ACGLN
    CD43 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50735-ACG
    CD43 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50735-ACR
    CD43 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50735-CF
    CD43 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50735-NF
    CD43 cDNA ORF Clone, Mouse, C-His tag MG50735-CH
    CD43 cDNA ORF Clone, Mouse, N-His tag MG50735-NH
    CD43 cDNA ORF Clone, Mouse, C-HA tag MG50735-CY
    CD43 cDNA ORF Clone, Mouse, N-HA tag MG50735-NY
    CD43 cDNA ORF Clone, Mouse, C-Myc tag MG50735-CM
    CD43 cDNA ORF Clone, Mouse, N-Myc tag MG50735-NM
    CD43 qPCR Primer Pairs, Mouse MP200710

    CD43 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CD43 cDNA ORF Clone in Cloning Vector, Human HG13108-G
    CD43 cDNA ORF Clone, Human, untagged HG13108-UT
    CD43 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13108-ACGLN
    CD43 cDNA ORF Clone, Human, C-GFPSpark® tag HG13108-ACG
    CD43 cDNA ORF Clone, Human, C-OFPSpark® tag HG13108-ACR
    CD43 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG13108-CF
    CD43 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13108-NF
    CD43 cDNA ORF Clone, Human, C-His tag HG13108-CH
    CD43 cDNA ORF Clone, Human, N-His tag HG13108-NH
    CD43 cDNA ORF Clone, Human, C-HA tag HG13108-CY
    CD43 cDNA ORF Clone, Human, N-HA tag HG13108-NY
    CD43 cDNA ORF Clone, Human, C-Myc tag HG13108-CM
    CD43 cDNA ORF Clone, Human, N-Myc tag HG13108-NM
    CD43 qPCR Primer Pairs, Human HP101730
    info info
    添加购物车成功! 添加购物车失败!