S100A16 qPCR Primer Pairs, Rat

Cat: RP300700
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S100A16 qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
S100A16 Background Information

S100A16 is a member of S100 protein super family that carries calcium-binding EF-hand motifs. S100 proteins are cell- and tissue-specific and are involved in many intra- and extracellular processes through interacting with specific target proteins. S100A16 expression was found to be astrocyte-specific. The S100A16 protein was found to accumulate within nucleoli and to translocate to the cytoplasm in response to Ca(2+) stimulation. The homodimeric structure of human S100A16 in the apo state has been obtained both in the solid state and in solution, resulting in good agreement between the structures with the exception of two loop regions. The homodimeric solution structure of human S100A16 was also calculated in the calcium(II)-bound form. Differently from most S100 proteins, the conformational rearrangement upon calcium binding is minor. Immunoprecipitation analysis revealed that S100A16 could physically interact with tumor suppressor protein p53, also a known inhibitor of adipogenesis. Overexpression or RNA interference-initiated reduction of S100A16 led to the inhibition or activation of the expression of p53-responsive genes, respectively. S100A16 protein is a novel adipogenesis-promoting factor.

Full Name
S100 calcium binding protein A16
References
  • Sturchler E, et al. (2006) S100A16, a novel calcium-binding protein of the EF-hand superfamily. J Biol Chem. 281(50): 38905-17.
  • Liu Y, et al. (2011) Identification of S100A16 as a Novel Adipogenesis Promoting Factor in 3T3-L1 Cells. Endocrinology. 152(3): 903-11.
  • Babini E, et al. (2011) Structural characterization of human S100A16, a low-affinity calcium binder. J Biol Inorg Chem. 16(2): 243-56.
  • S100A16 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    S100A16 cDNA ORF Clone in Cloning Vector, Rat RG80736-U
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    S100A16 cDNA ORF Clone, Rat, C-HA tag RG80736-CY
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    S100A16 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    S100A16 cDNA ORF Clone in Cloning Vector, Mouse MG51916-U
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    S100A16 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51916-ACGLN
    S100A16 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51916-ACG
    S100A16 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51916-ANG
    S100A16 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51916-ACR
    S100A16 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51916-ANR
    S100A16 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51916-CF
    S100A16 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51916-NF
    S100A16 cDNA ORF Clone, Mouse, C-His tag MG51916-CH
    S100A16 cDNA ORF Clone, Mouse, N-His tag MG51916-NH
    S100A16 cDNA ORF Clone, Mouse, C-HA tag MG51916-CY
    S100A16 cDNA ORF Clone, Mouse, N-HA tag MG51916-NY
    S100A16 cDNA ORF Clone, Mouse, C-Myc tag MG51916-CM
    S100A16 cDNA ORF Clone, Mouse, N-Myc tag MG51916-NM

    S100A16 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    S100A16 cDNA ORF Clone in Cloning Vector, Human HG11137-U
    S100A16 cDNA ORF Clone, Human, untagged HG11137-UT
    S100A16 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11137-ACGLN
    S100A16 cDNA ORF Clone, Human, C-GFPSpark® tag HG11137-ACG
    S100A16 cDNA ORF Clone, Human, N-GFPSpark® tag HG11137-ANG
    S100A16 cDNA ORF Clone, Human, C-OFPSpark® tag HG11137-ACR
    S100A16 cDNA ORF Clone, Human, N-OFPSpark® tag HG11137-ANR
    S100A16 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11137-CF
    S100A16 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11137-NF
    S100A16 cDNA ORF Clone, Human, C-His tag HG11137-CH
    S100A16 cDNA ORF Clone, Human, N-His tag HG11137-NH
    S100A16 cDNA ORF Clone, Human, C-HA tag HG11137-CY
    S100A16 cDNA ORF Clone, Human, N-HA tag HG11137-NY
    S100A16 cDNA ORF Clone, Human, C-Myc tag HG11137-CM
    S100A16 cDNA ORF Clone, Human, N-Myc tag HG11137-NM
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