|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat REG3A ORF mammalian expression plasmid, C-GFPSpark tag||RG80258-ACG|
|Rat REG3A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80258-ACR|
|Rat REG3A ORF mammalian expression plasmid, C-Flag tag||RG80258-CF|
|Rat REG3A ORF mammalian expression plasmid, C-His tag||RG80258-CH|
|Rat REG3A ORF mammalian expression plasmid, C-Myc tag||RG80258-CM|
|Rat REG3A ORF mammalian expression plasmid, C-HA tag||RG80258-CY|
|Rat REG3A Gene cDNA clone plasmid||RG80258-G|
|Rat REG3A ORF mammalian expression plasmid, N-Flag tag||RG80258-NF|
|Rat REG3A ORF mammalian expression plasmid, N-His tag||RG80258-NH|
|Rat REG3A ORF mammalian expression plasmid, N-Myc tag||RG80258-NM|
|Rat REG3A ORF mammalian expression plasmid, N-HA tag||RG80258-NY|
|Rat REG3A natural ORF mammalian expression plasmid||RG80258-UT|
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Regenerating islet-derived protein 3-alpha, also known as Regenerating islet-derived protein III-alpha, REG-3-alpha, REG3A, and HIP, is secreted protein which contains one C-type lectin domain. REG3A is constitutively expressed in intestine, and is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It is overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. REG3A is also a stress protein involved in the control of bacterial proliferation. REG3A is down-regulated in most primary human gastric cancer cells, and might be useful in the diagnosis of gastric cancer. Additionally, REG3A is a target of beta-catenin signaling in Huh7 hepatoma cells. The REG1A and REG3A are downstream targets of the Wnt pathway during liver tumorigenesis.