|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat RAMP3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80690-ACG|
|Rat RAMP3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80690-ACR|
|Rat RAMP3 ORF mammalian expression plasmid, C-Flag tag||RG80690-CF|
|Rat RAMP3 ORF mammalian expression plasmid, C-His tag||RG80690-CH|
|Rat RAMP3 ORF mammalian expression plasmid, C-Myc tag||RG80690-CM|
|Rat RAMP3 ORF mammalian expression plasmid, C-HA tag||RG80690-CY|
|Rat RAMP3 ORF mammalian expression plasmid, N-Flag tag||RG80690-NF|
|Rat RAMP3 ORF mammalian expression plasmid, N-His tag||RG80690-NH|
|Rat RAMP3 ORF mammalian expression plasmid, N-Myc tag||RG80690-NM|
|Rat RAMP3 ORF mammalian expression plasmid, N-HA tag||RG80690-NY|
|Rat RAMP3 Gene cDNA clone plasmid||RG80690-U|
|Rat RAMP3 natural ORF mammalian expression plasmid||RG80690-UT|
|Learn more about expression Vectors|
RAMP3 belongs to the RAMP family. Members of this family are single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs have a wide biological distribution; high concentrations are found in the brain, lung, liver, heart and spleen with lower expression levels present in the testes, gastrointestinal tract and thyroid. RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. They are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of RAMP3 protein, CRLR functions as an adrenomedullin receptor.