PRMT5 qPCR Primer Pairs, Rat

Cat: RP301220
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PRMT5 qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
PRMT5 Background Information

Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally, it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.

Full Name
protein arginine methyltransferase 5
References
  • Rho. J. et al., 2001, J.Biol. Chem. 276: 11393-11401.
  • Fabbrizio, E.et al., 2002, EMBO.Rep. 3: 641-645.
  • Azzouz, T.N. et al., 2005, J.Biol. Chem. 280: 34435-34440.
  • Pal, S., et al., 2004, Mol. Cell. Biol. 24:9630-9645.
  • Herrmann, FJ. et al., 2009, Cell Sci. 122 (Pt 5): 667-77.
  • PRMT5 qPCR Primer Pairs, Rat, Related Products

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    PRMT5 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
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    PRMT5 cDNA ORF Clone, Mouse, C-His tag MG50936-CH
    PRMT5 cDNA ORF Clone, Mouse, N-His tag MG50936-NH
    PRMT5 cDNA ORF Clone, Mouse, C-HA tag MG50936-CY
    PRMT5 cDNA ORF Clone, Mouse, N-HA tag MG50936-NY
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    PRMT5 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    PRMT5 cDNA ORF Clone in Cloning Vector, Human HG11074-M
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    PRMT5 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11074-ACGLN
    PRMT5 cDNA ORF Clone, Human, N-GFPSpark® tag HG11074-ANG
    PRMT5 cDNA ORF Clone, Human, C-OFPSpark® tag HG11074-ACR
    PRMT5 cDNA ORF Clone, Human, N-OFPSpark® tag HG11074-ANR
    PRMT5 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11074-CF
    PRMT5 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11074-NF
    PRMT5 cDNA ORF Clone, Human, C-His tag HG11074-CH
    PRMT5 cDNA ORF Clone, Human, N-His tag HG11074-NH
    PRMT5 cDNA ORF Clone, Human, C-HA tag HG11074-CY
    PRMT5 cDNA ORF Clone, Human, N-HA tag HG11074-NY
    PRMT5 cDNA ORF Clone, Human, C-Myc tag HG11074-CM
    PRMT5 cDNA ORF Clone, Human, N-Myc tag HG11074-NM
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