|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat Neuropilin-1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80056-ACG|
|Rat Neuropilin-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80056-ACR|
|Rat Neuropilin-1 ORF mammalian expression plasmid, C-Flag tag||RG80056-CF|
|Rat Neuropilin-1 ORF mammalian expression plasmid, C-His tag||RG80056-CH|
|Rat Neuropilin-1 ORF mammalian expression plasmid, C-Myc tag||RG80056-CM|
|Rat Neuropilin-1 ORF mammalian expression plasmid, C-HA tag||RG80056-CY|
|Rat Neuropilin-1 Gene cDNA clone plasmid||RG80056-M|
|Rat Neuropilin-1 ORF mammalian expression plasmid, N-Flag tag||RG80056-NF|
|Rat Neuropilin-1 ORF mammalian expression plasmid, N-His tag||RG80056-NH|
|Rat Neuropilin-1 ORF mammalian expression plasmid, N-Myc tag||RG80056-NM|
|Rat Neuropilin-1 ORF mammalian expression plasmid, N-HA tag||RG80056-NY|
|Rat Neuropilin-1 natural ORF mammalian expression plasmid||RG80056-UT|
|Learn more about expression Vectors|
Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologues, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.