|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat NCR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80378-ACG|
|Rat NCR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80378-ACR|
|Rat NCR1 ORF mammalian expression plasmid, C-Flag tag||RG80378-CF|
|Rat NCR1 ORF mammalian expression plasmid, C-His tag||RG80378-CH|
|Rat NCR1 ORF mammalian expression plasmid, C-Myc tag||RG80378-CM|
|Rat NCR1 ORF mammalian expression plasmid, C-HA tag||RG80378-CY|
|Rat NCR1 Gene cDNA clone plasmid||RG80378-G|
|Rat NCR1 ORF mammalian expression plasmid, N-Flag tag||RG80378-NF|
|Rat NCR1 ORF mammalian expression plasmid, N-His tag||RG80378-NH|
|Rat NCR1 ORF mammalian expression plasmid, N-Myc tag||RG80378-NM|
|Rat NCR1 ORF mammalian expression plasmid, N-HA tag||RG80378-NY|
|Rat NCR1 natural ORF mammalian expression plasmid||RG80378-UT|
|Learn more about expression Vectors|
NCR1, also known as NK-p46 and CD335, is a natural cytotoxicity receptor(NCR). NCRs are type I transmembrane proteins with 1-2 extracellular immunoglobulin domains, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. All are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium.