|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat MSR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80363-ACG|
|Rat MSR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80363-ACR|
|Rat MSR1 ORF mammalian expression plasmid, C-Flag tag||RG80363-CF|
|Rat MSR1 ORF mammalian expression plasmid, C-His tag||RG80363-CH|
|Rat MSR1 ORF mammalian expression plasmid, C-Myc tag||RG80363-CM|
|Rat MSR1 ORF mammalian expression plasmid, C-HA tag||RG80363-CY|
|Rat MSR1 Gene cDNA clone plasmid||RG80363-G|
|Rat MSR1 ORF mammalian expression plasmid, N-Flag tag||RG80363-NF|
|Rat MSR1 ORF mammalian expression plasmid, N-His tag||RG80363-NH|
|Rat MSR1 ORF mammalian expression plasmid, N-Myc tag||RG80363-NM|
|Rat MSR1 ORF mammalian expression plasmid, N-HA tag||RG80363-NY|
|Rat MSR1 natural ORF mammalian expression plasmid||RG80363-UT|
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Macrophage scavenger receptor types I and II, also known as Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1, CD204, MSR1 and SCARA1, is a single-pass type I I membrane protein which contains one collagen-like domain and one SRCR domain. Macrophages are distributed in all peripheral tissues and play a critical role in the first line of the innate immune defenses against bacterial infection by phagocytosis of bacterial pathogens through the macrophage scavenger receptor 1 (MSR1). MSR1 / SCARA1 is one of the membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). MSR1 / SCARA1 is also involved in chronic inflammation which is a risk factor for prostate cancer. MSR1 1 gene was identified as a candidate susceptibility gene for hereditary prostate cancer and as a risk factor for sporadic prostate cancer.