methionyl aminopeptidase 2 qPCR Primer Pairs, Rat

Cat: RP301143
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methionyl aminopeptidase 2 qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
methionyl aminopeptidase 2 Background Information

METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.

Full Name
methionyl aminopeptidase 2
Research Areas
References
  • Bennett, et al. (1997) EPR Studies on the Mono- and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33.
  • Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92.
  • Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.
  • methionyl aminopeptidase 2 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    methionyl aminopeptidase 2 cDNA ORF Clone in Cloning Vector, Rat RG81187-U
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, untagged RG81187-UT
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, C-GFPSpark® tag RG81187-ACG
    methionyl aminopeptidase 2 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81187-ACGLN
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, N-GFPSpark® tag RG81187-ANG
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, C-OFPSpark® tag RG81187-ACR
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, N-OFPSpark® tag RG81187-ANR
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG81187-CF
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG81187-NF
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, C-His tag RG81187-CH
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, N-His tag RG81187-NH
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, C-HA tag RG81187-CY
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, N-HA tag RG81187-NY
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, C-Myc tag RG81187-CM
    methionyl aminopeptidase 2 cDNA ORF Clone, Rat, N-Myc tag RG81187-NM

    methionyl aminopeptidase 2 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    methionyl aminopeptidase 2 cDNA ORF Clone in Cloning Vector, Mouse MG50528-M
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, untagged MG50528-UT
    methionyl aminopeptidase 2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50528-ACGLN
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50528-ACG
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50528-ANG
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50528-ACR
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50528-ANR
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50528-CF
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50528-NF
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, C-His tag MG50528-CH
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, N-His tag MG50528-NH
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, C-HA tag MG50528-CY
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, N-HA tag MG50528-NY
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, C-Myc tag MG50528-CM
    methionyl aminopeptidase 2 cDNA ORF Clone, Mouse, N-Myc tag MG50528-NM

    methionyl aminopeptidase 2 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    methionyl aminopeptidase 2 cDNA ORF Clone in Cloning Vector, Human HG10245-M
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, untagged HG10245-UT
    methionyl aminopeptidase 2 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10245-ACGLN
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-GFPSpark® tag HG10245-ACG
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, N-GFPSpark® tag HG10245-ANG
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-OFPSpark® tag HG10245-ACR
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, N-OFPSpark® tag HG10245-ANR
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10245-CF
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10245-NF
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-His tag HG10245-CH
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, N-His tag HG10245-NH
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-HA tag HG10245-CY
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, N-HA tag HG10245-NY
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, C-Myc tag HG10245-CM
    methionyl aminopeptidase 2 cDNA ORF Clone, Human, N-Myc tag HG10245-NM
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